Fatty acid tail length and saturation affect interactions with hASIC3. (A) Occupancies of palmitic (C16:0), arachidonic (C20:4), and docosahexaenoic (C22:6) acid at the TM1 helix. (B) Occupancies of palmitic, arachidonic, and docosahexaenoic acid at the TM2 helix. The occupancy data is averaged over three chains from three repeats simulated for 30 µs each, and standard deviations are shown with black lines. Bars represent averages while points represent individual samples.