Mutations to three key residues change AA interaction pattern. (A) AA occupancy comparison between WT hASIC3 (cyan) and triple mutant hASIC3 (orange). The occupancy data is averaged over three chains from three repeats simulated for 30 µs each, and standard deviations are shown with black lines. Bars show averages while points show individual samples. Residue numbering corresponds to hASIC3. (B) AA occupancy comparison between WT hASIC3 (cyan) and triple mutant hASIC1a (orange). Mutated residues are indicated with arrowheads. Residue numbering corresponds to hASIC1a.