Interaction site residues differ between subunit types. (A) Alignment of the upper part of TM1 for ASIC1 and ASIC3 subtypes from different species. (B) Representative binding pose (CG representation) of AA at conserved ASIC3 residues determined by PyLipID clustering analysis, as described in Song et al. (2022). The red bead near R65 represents the charged headgroup as defined by the COO bead.