AA participates in longer interactions with hASIC3 open state compared to resting state. (A) Occupancies of AA at TM1 residues for the hASIC3 open state in cyan and the resting state in light blue. (B) Average interaction duration of AA at TM1 residues. All values calculated as averages over three chains in three repeats of 30 µs (i.e., each value is an average of nine samples). Standard deviations are shown as black lines. Bars represent averages, while points represent individual samples.