Analysis of contractile parameters over an Ani9 concentration response in IALVs from Ip3r1fl/fl and Ip3r1ismKO mice. (A and B) Representative diameter traces over 1-min periods in IALVs from C57BL6J controls (A) and Ip3r1ismKO (B) mice in response to 0.1, 0.3, 1, 3, and 6 μΜ Ani9. (C–E) Statistical summary of contractile parameters of (C) frequency, (D) amplitude, and (E) tone. n = 8 Ip3r1fl/fl and n = 8 Ip3r1ismKO IALVs with mean and SEM shown.