Figure 8.
Membrane potential recordings from IALVs from Ip3r1fl/fl and Ip3r1ismKO mice. Membrane potential was recorded in pressurized and contracting IALVs from Ip3r1fl/fl and Ip3r1ismKO mice using sharp electrodes and APs were analyzed. (A) Representative membrane potential recordings (30 s) from IALVs isolated from Ip3r1fl/fl (black lines) and Ip3r1ismKO (red lines) mice reveal significant differences in the observed diastolic depolarization and plateau duration. (B) Zoom of the 4 s window outlined by the dashed box in A. (C–K) Analysis summary of membrane potential and AP characteristics including (C) AP frequency, (D) diastolic depolarization rate mv/s, (E) time to reach threshold, (F) min Vm, (G) threshold Vm, (H) peak AP Vm, (I) plateau Vm, (J) time over threshold, and (K) sharp upstroke τ value. n = 4 Ip3r1fl/fl and n = 10 Ip3r1ismKO IALVs with mean and SEM shown.

Membrane potential recordings from IALVs from Ip3r1fl/fl and Ip3r1ismKO mice. Membrane potential was recorded in pressurized and contracting IALVs from Ip3r1fl/fl and Ip3r1ismKO mice using sharp electrodes and APs were analyzed. (A) Representative membrane potential recordings (30 s) from IALVs isolated from Ip3r1fl/fl (black lines) and Ip3r1ismKO (red lines) mice reveal significant differences in the observed diastolic depolarization and plateau duration. (B) Zoom of the 4 s window outlined by the dashed box in A. (C–K) Analysis summary of membrane potential and AP characteristics including (C) AP frequency, (D) diastolic depolarization rate mv/s, (E) time to reach threshold, (F) min Vm, (G) threshold Vm, (H) peak AP Vm, (I) plateau Vm, (J) time over threshold, and (K) sharp upstroke τ value. n = 4 Ip3r1fl/fl and n = 10 Ip3r1ismKO IALVs with mean and SEM shown.

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