Analysis of contractile parameters in IALVs from Ip3r1fl/fl and Ip3r1ismKO mice when challenged with a pressure response using a bubbled bicarbonate-based buffer instead of our HEPES buffered Krebs. (A) Comparison of frequency achieved at P = 3 cmH₂O in HEPES buffered Krebs and P = 3 cmH₂O in the 21% O₂–5% CO₂ bubbled bicarbonate PSS buffer pre- and post-pressure step. (B–G) Summary of the contractile data over the pressure response in bubbled bicarb PSS buffer including (B) frequency, (C) amplitude, (D) tone, (E) EF, and (F) FPF across each pressure. n = 4 Ip3r1fl/fl and n = 5 Ip3r1ismKO IALVs with mean and SEM shown.