Representative inner diameter traces of isolated IALVs subjected to our isobaric myography pressure step protocol. (A and B) Representative diameter traces over 1-min periods in IALVs from Ip3r1fl/fl controls (A) and Ip3r1ismKO (B) mice across the pressure range 0.5, 1, 2, 3, 5, and finally 8 cmH₂O. (C) Overlaid diameter traces of IALVs from Ip3r1fl/fl controls (solid line) and Ip3r1ismKO (dashed line) mice at 3 cmH₂O. (D) Control Ip3r1fl/fl IALVs develop myogenic constriction upon stepping pressure from 3 to 5 cmH₂O, dashed line represents acute maximum diastolic diameter after the pressure step. (E)Ip3r1ismKO vessels do not develop myogenic constriction to the 3 to 5 cmH₂O pressure step. Arrows indicate pressure change.