Figure S3.
Fraction of mobile filaments upon IR laser irradiation at pCa 9. (A) Relationship between temperature and the fraction of mobile skeletal thin filaments (red closed circles) or cardiac thin filaments (blue closed circles) on skeletal myosin. Regression analysis was performed based on the Hill equation (see Materials and methods). The T50 values were 32.5°C and 28.5°C for skeletal and cardiac thin filaments, respectively. (B) Relationship between temperature and the fraction of mobile skeletal thin filaments (red closed squares) or cardiac thin filaments (blue closed squares) on β-cardiac myosin. The T50 values were 34.1°C and 30.3°C for skeletal and cardiac thin filaments, respectively. See Table S7 for details.
