Time trajectories of individual residues, showing continuous deviation from the fourfold symmetry. (a) Diagonal distance difference of each residue for the simulations with (left) or without (right) PIP₂. The range of difference is shown in the color bar. (b) Diagonal angle difference from 90° of each residue for the simulations with (left) or without (right) PIP₂. The range of difference is shown in the color bar. T, residues in the TMD; C, residues in the CTD.