Figure S10. Model-predicted uptakes of... | Rockefeller University Press

Figure S10.

$Model-predicted uptakes of radioactive Arg+into AdiC-embedded lipid vesicles compared with the observed and theoretical maximum values. (A) The model-predicted fractions of radioactive Arg+ (mean ± σ) accumulated inside the vesicles for individual indicated durations were obtained from the simulation with the model (Fig. S6), as shown in Fig. 11 A (see Results, and supplemental text at the end of the PDF), under the initial conditions that the bathing solution always contained 50 µM radioactive Arg+, whereas the vesicles contained 5 mM Agm2+ (closed blue circles), 5 mM Arg+ (closed green circles), or 5 mM functionally inert D-Arg+ (closed black circles), compared with the previously Fmaxobs for the corresponding conditions indicated by the solid lines in the respective color (Fang et al., 2007). The dashed line at the top indicates the theoretical Fmax for an obligatory 1:1 exchange under the initial conditions that the bathing solution always contained 50 µM radioactive Arg+, whereas the vesicles contained 5 mM Agm2+ or Arg+ (Eq. 10). (B) The model-predicted time-dependent fractions of radioactive Arg+ uptake (mean ± σ) were obtained from the simulation with the model after omitting the apo path for confirmational transitions, under the initial condition that the bathing solution always contained 50 µM radioactive Arg+, whereas the vesicles contained 5 mM Arg+ (open black squares) or Agm2+ (open maroon squares), compared with the theoretical Fmax indicated by the black dashed line.$

Model-predicted uptakes of radioactive Arg ⁺ into AdiC-embedded lipid vesicles compared with the observed and theoretical maximum values. (A) The model-predicted fractions of radioactive Arg⁺ (mean ± σ) accumulated inside the vesicles for individual indicated durations were obtained from the simulation with the model (Fig. S6), as shown in Fig. 11 A (see Results, and supplemental text at the end of the PDF), under the initial conditions that the bathing solution always contained 50 µM radioactive Arg⁺, whereas the vesicles contained 5 mM Agm²⁺ (closed blue circles), 5 mM Arg⁺ (closed green circles), or 5 mM functionally inert D-Arg⁺ (closed black circles), compared with the previously $F_{\max}^{o b s}$ for the corresponding conditions indicated by the solid lines in the respective color (Fang et al., 2007). The dashed line at the top indicates the theoretical F_max for an obligatory 1:1 exchange under the initial conditions that the bathing solution always contained 50 µM radioactive Arg⁺, whereas the vesicles contained 5 mM Agm²⁺ or Arg⁺ (Eq. 10). (B) The model-predicted time-dependent fractions of radioactive Arg⁺ uptake (mean ± σ) were obtained from the simulation with the model after omitting the apo path for confirmational transitions, under the initial condition that the bathing solution always contained 50 µM radioactive Arg⁺, whereas the vesicles contained 5 mM Arg⁺ (open black squares) or Agm²⁺ (open maroon squares), compared with the theoretical F_max indicated by the black dashed line.