Dependence of probabilities of state-to-state transitions on the concentration of ligands. (A and B) Probabilities of state-to-state transitions plotted against the concentration of Arg⁺ (A) or Agm²⁺ (B). All data are presented as mean ± SEM, and their representing symbols are color coded for states. The lines superimposed on the data correspond to fits of the respective groups of data to an equation in the same form as Eq. 3. Fitted parameter values are given in Tables S1, S2, S3, and S4.