Ligand dependence of the probabilities of conformational states and the diagram of a conformational state model of AdiC. (A) The probabilities of individual four states (mean ± SEM, n = 945–3,187) are plotted against the Agm²⁺ concentration on a logarithm scale, along with those previously obtained with Arg⁺ on the right (Zhou et al., 2023). The curve superimposed on the data for a given conformation represents the predicted relation by the kinetic model (Fig. 6). (B) An eight-state model that accounts for the observed conformational behaviors of AdiC: four apo states and four Arg⁺- or Agm²⁺-bound states.