Niclosamide causes vasoconstriction by potentiation of TMEM16A. (A) Representative pressure myograph images of the mesenteric artery in response to sequential application of compounds, including 5 µM niclosamide (Niclo), 10 µM Ani9 + 5 µM Niclo, 10 µM ACh, and 10 μM PE. (B) Relative outer diameter changes of the mesenteric artery in A. (C) Comparison of drug-induced mesenteric artery dilation. Data was calculated as relaxation percentages normalized to the ACh-induced complete vasodilation (see Materials and methods for details). Statistical analysis was done by one-way ANOVA followed by Tukey’s multiple comparisons test. **: P < 0.01, n = 5 individual mesenteric arteries from three male mice.