Niclosamide potentiates TMEM16A overexpressed in HEK293T cells and regulates cell volume. (A and B) Left: Representative images of the cell volume changes of the voltage-clamped HEK293T cells overexpressing TMEM16A-mCherry in the absence (A) and presence (B) of 5 µM niclosamide. Right: Comparison of cell volume changes before and after niclosamide. Statistical analysis was done with paired Student’s t-test (ns means no significance, **** means P < 0.0001, n = 7 for A and n = 9 for B). (C) Left: Representative images of the cell volume changes of the voltage-clamped HEK293T cells without TMEM16A overexpression treated with 5 µM niclosamide. Right: Comparison of cell volume changes before and after niclosamide. Statistical analysis was done with Student’s t-test (ns means no significance, n = 7). All experiments were done under whole-cell patch clamp configuration with a holding potential at −60 mV and intracellular free Ca2+ of 0.5 µM.
