Niclosamide potentiates TMEM16A “ac” splicing isoform overexpressed in HEK293T cells. (A) Representative whole-cell current traces induced by a voltage-step protocol from −100 to +140 mV with 20-mV increments and the holding potential (HP) at −60 mV in the absence (control) or presence of 5 µM niclosamide. Intracellular solution contains 0.5 µM free Ca²⁺. (B)G-V relationship of TMEM16A currents recorded in A. All tail currents (*) were normalized to the tail current elicited by a +140 mV depolarization pulse in bath solution (black *). Error bars represent SEM, n = 4.