Confocal micrographs of fixed adult murine ventricular cardiomyocytes treated with 1 µM ISO for 3 min and immunostained with anti-Flag antibody to detect Flag-Rad localization. (A) Cells were isolated from the hearts of transgenic mice expressing full-length Flag-Rad (N = 6 mice, n = 8 cells displayed) or Flag-RadΔCT (N = 6 mice, n = 8 cells displayed), representative of 6 and 6 mice, 29 and 32 cells, and 64 and 62 images. Scale bar, 5 µm; magnification is the same for all images. (B) Fundamental peak power of anti-Flag immunostaining that represents a signal with periodicity consistent with t-tubular expression of both genotypes and ±ISO. In A and Fig. 5 B, the data is plotted on a linear y-axis here instead of log10. (C) The individual cell data points are shown that were averaged for the mouse data, both in linear and log10 scale. A line in both graphs at FFT Power 10 is denoted, corresponding to the proportions of cells over 10 shown in Fig. 2 C and Fig. 5 E. Note that for cells, the 75th percentile of both Flag-RadΔCT groups are below this threshold of 10 FFT Power.
