Figure S5.
Acute β-adrenergic stimulation does not induce NaV1.5 clustering to intercalated disc region. Adult rat ventricular myocytes were incubated under the control conditions or with isoproterenol (ISO, 100 nM) for 15 min before the experiment. Myocytes from the same heart were cultured under the control conditions or with 8CPT-cAMP (100 μM)/okadaic acid (100 nM) for 12 h before the experiment. (A) Representative Airyscan images of NaV1.5 immunofluorescence from the four groups of myocytes. (B) Bar graph (mean + SE) and individual data points of NaV1.5 enrichment at intercalated discs. The numbers of myocytes analyzed are in parentheses. Listed P values are from t test between PKA and CON myocytes. Scale bars in A are 10 μm.

Acute β-adrenergic stimulation does not induce Na V 1.5 clustering to intercalated disc region. Adult rat ventricular myocytes were incubated under the control conditions or with isoproterenol (ISO, 100 nM) for 15 min before the experiment. Myocytes from the same heart were cultured under the control conditions or with 8CPT-cAMP (100 μM)/okadaic acid (100 nM) for 12 h before the experiment. (A) Representative Airyscan images of NaV1.5 immunofluorescence from the four groups of myocytes. (B) Bar graph (mean + SE) and individual data points of NaV1.5 enrichment at intercalated discs. The numbers of myocytes analyzed are in parentheses. Listed P values are from t test between PKA and CON myocytes. Scale bars in A are 10 μm.

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