EB1 and β-Tub coimmunoprecipitation was reduced, while Na _V 1.5 and β-Tub coimmunoprecipitation (co-IP) trended higher with persistent PKA activation. Experiments were done in HEK293 cells expressing GFP-Na_V1.5 and native EB1 and β-Tub, without or with PKA activation (4 h). Cells were lysed in 1% Triton lysis buffer, and WCLs were subject to immunoprecipitation with GFP rabbit or EB1 rat Ab and protein A/G beads ([+] IP). “(−) IPs” were negative control (WCL incubated with protein A/G beads without immunoprecipitating antibodies). (A) Representative immunoblot images probed with Abs listed on the left. The EB1 rabbit Ab IB image of EB1 rat Ab IP is modified from the same experiment image shown in Fig. 9 A. (B) Top: Degrees of β-Tub co-IP with GFP-Na_V1.5 or EB1 from CON and PKA cells quantified by β-Tub band intensity in (+) IP lane divided by that in WCL ([+] IP/WCL). Bottom: PKA:CON ratio of degree of β-Tub co-IP with GFP-Na_V1.5 or with EB1. The dotted line denotes PKA:CON of 1. Numbers in parentheses are those of independent experiments. Listed P values are from t tests against null hypothesis. Source data are available for this figure: SourceData F11.