Persistent PKA activation for 12 h caused an enrichment of Na V 1.5, EB1, and β-Tub immunofluorescence along the lateral surface and at intercalated discs of ventricular myocytes. (A) Airyscan images of NaV1.5, EB1, β-Tub, and nCad immunofluorescence in CON and PKA myocytes. (B) Box plots of whole myocyte pixel content (normalized to the mean values of CON myocytes), enrichment of immunofluorescence along the lateral surface, and enrichment of immunofluorescence at intercalated disc regions. Data were pooled from five independent experiments, with 40–70 myocytes per group. Dotted line indicates CON mean (top) or no enrichment (middle and bottom). Listed P values are from t tests of PKA versus CON myocytes.
