Colocalization of α-tubulin and β-tubulin (α- and β-Tub, respectively) immunofluorescence in rat ventricular myocytes. Shown are Airyscan images α-Tub and β-Tub immunofluorescence (detected by α-Tub rat Ab and β-Tub mouse Ab) and their merge in control and PKA myocytes (PKA activation 12 h). Images were obtained in a z-plane close to the myocyte’s surface.