The EB1 transcript was upregulated, while Na _V 1.5 transcript was downregulated in PKA versus CON myocytes. Total RNAs were extracted from CON and PKA myocytes (incubation time 15 h, five independent experiments) with TRIZOL reagents. After confirming high sample quality (RNA integrity number >7), the RNA concentrations were adjusted to 100 ng/µl and used for two groups of experiments. Group 1 was RNA-seq experiment: mRNA library construction followed by next-generation sequencing (VCU Genomics Core). Raw FASTQ sequences of individual samples were inspected for quality using the tool FastQC and then merged with multiQC. PE 100 bp reads were aligned to the rat primary genome assembly (mRatBN7.2, version 110). Raw counts were normalized between and within samples, using EdgeR’s calcNormFactors scaling factor of trimmed mean of M-values (TMM), that took into account of variations in library size, sequencing depth, and gene lengths. Group 2 was quantitative RT-PCR experiment: mRNA was reverse transcribed followed by PCR reactions using the following primer pairs that cross exon-intron boundaries: (a) EB1: forward 5′-TGT​CGC​TCC​AGC​TTT​GAG​TA-3′, reverse 5′-AGC​AGC​TTC​GTC​ATC​TCC​AT-3′, (b) Na_V1.5: forward 5′-TCA​ATG​ACC​CAG​CCA​ATT​ACC​T-3′, reverse 5′-CCC​GGC​ATC​AGA​GCT​GTT-3'. House-keep transcript GAPDH was included in the qPCR reactions. Data of TMM (RNA-seq) or GAPDH (qPCR) normalized transcript levels are presented as individual data points of CON-PKA pairs. Each pair is connected by a dashed line for visual effect clarification. The P values are from paired t tests between CON and PKA data points.