Distribution of the bound heads in the F-actin–HMM and cTFs–HMM complexes . (a–h) Kymograph images of the F-actin–HMM (a–d) and cTFs–HMM (e–h) complexes at the different ATP concentrations. Scan area and scan rates of the top HS-AFM images for F-actin–HMM complex: (a) 150 × 75 nm², 80 × 40 pixels², 10 fps; (b) 150 × 90 nm², 80 × 48 pixels², 20 fps; (c) 200 × 120 nm², 80 × 40 pixels², 10 fps; (d) 200 × 120 nm², 80 × 40 pixels², 12.5 fps. Horizontal scale bars, 30 nm; vertical scale bars in kymographs, 20 ms. Scan area and scan rates of the top HS-AFM images for cTFs–HMM complex at the activating conditions: (e) 120 × 120 nm², 200 × 200 pixels², 6.7 fps; (f) 120 × 120 nm², 200 × 200 pixels², 2 fps; (g) 150 × 75 nm², 80 × 40 pixels², 6.7 fps; (h) 200 × 200 nm², 120 × 120 pixels², 2 fps. Horizontal scale bars, 30 nm; vertical scale bars in kymographs, 50 ms (e and f) and 100 ms (g and h). The dashed yellow line indicated the initial position to create a kymograph image.