Figure S3.
Data analysis pipeline. (a) The TrackMate analysis with Z-discs (left) and ATP localization events (right) as is seen during the analysis. Purple circles identify Z-disc tracked spots and multicoloured dots label Cy3-ATP tracks. (b) The combined data from both TrackMate analyses plotted with triangles representing the Z-disc positions and X marking Cy3-ATP localizations. A line of best fit was drawn through the tilted Z-disc and used to correct the tilt of that one sarcomere. This process was repeated for every sarcomere analyzed. (c) Corrected Z-disc and ATP localization data, with the dotted line showing the overlay of the axis with b; the aligned centre for the leftmost Z-disc becomes 0 on the x axis for c. All scale bars indicate 2 µm.

Data analysis pipeline. (a) The TrackMate analysis with Z-discs (left) and ATP localization events (right) as is seen during the analysis. Purple circles identify Z-disc tracked spots and multicoloured dots label Cy3-ATP tracks. (b) The combined data from both TrackMate analyses plotted with triangles representing the Z-disc positions and X marking Cy3-ATP localizations. A line of best fit was drawn through the tilted Z-disc and used to correct the tilt of that one sarcomere. This process was repeated for every sarcomere analyzed. (c) Corrected Z-disc and ATP localization data, with the dotted line showing the overlay of the axis with b; the aligned centre for the leftmost Z-disc becomes 0 on the x axis for c. All scale bars indicate 2 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal