Figure S11. Synaptic weight updates when... | Rockefeller University Press

Figure S11.

Synaptic weight updates when considering Ca2+in terms of ions or concentration for the filopodia-shaped spines. (a) Spatial plots illustrating Ca2+ localization at 15 and 30 ms for a filopodia-shaped spine with volume 0.138 μm3. The number above each geometry corresponds to the number of Ca2+ in that frame. Two random seeds are shown. Scale bars, 2 μm. (b) Mean (solid) and SD (shaded area) of Ca2+ transients across 50 simulations for each of the three filopodia-shaped spine sizes (0.017, 0.058, and 0.138 μm3). (c) Synaptic weight prediction for each of the filopodia geometries calculated as a function of total calcium ions. We fitted the trends using a linear function of volume-to-surface-area ratio; r2 = 0.3594 for the ion fit. (d) Mean of the calcium transients for each filopodia-shaped spine size converted to concentration. (e) Synaptic weight prediction for each of the filopodia geometries calculated as a function of calcium concentration. We fitted the trends using a linear function of volume-to-surface-area ratio; r2 = 0.1143 for the concentration fit. We found statistically significant differences between the first and third spines and between the second and third spines; P12 = 0.1301; P23 = 2.2567 × 10−4; P13 = 1.1347 × 10−4 from two-tailed t test, where 1, 2, and 3 correspond to the spines in increasing volume.

Synaptic weight updates when considering Ca ²⁺ in terms of ions or concentration for the filopodia-shaped spines. (a) Spatial plots illustrating Ca²⁺ localization at 15 and 30 ms for a filopodia-shaped spine with volume 0.138 μm³. The number above each geometry corresponds to the number of Ca²⁺ in that frame. Two random seeds are shown. Scale bars, 2 μm. (b) Mean (solid) and SD (shaded area) of Ca²⁺ transients across 50 simulations for each of the three filopodia-shaped spine sizes (0.017, 0.058, and 0.138 μm³). (c) Synaptic weight prediction for each of the filopodia geometries calculated as a function of total calcium ions. We fitted the trends using a linear function of volume-to-surface-area ratio; r² = 0.3594 for the ion fit. (d) Mean of the calcium transients for each filopodia-shaped spine size converted to concentration. (e) Synaptic weight prediction for each of the filopodia geometries calculated as a function of calcium concentration. We fitted the trends using a linear function of volume-to-surface-area ratio; r² = 0.1143 for the concentration fit. We found statistically significant differences between the first and third spines and between the second and third spines; P₁₂ = 0.1301; P₂₃ = 2.2567 × 10⁻⁴; P₁₃ = 1.1347 × 10⁻⁴ from two-tailed t test, where 1, 2, and 3 correspond to the spines in increasing volume.