![Properties of the reconstituted DICR platform. (A) Typical results of R-CEPIA1er fluorescence in cells infected with 5 BV (left), BV without Cav1.1 (center), and 5 BV plus γ1 (right). (B) [K+] dependence of R-CEPIA1er fluorescence (F/F0) in 5 BV (black), −Cav1.1 (red), and −JP2 (dark blue). Data are means ± SD (n = 9, N = 3 for 5 BV; n = 6, N = 3 for −Cav1.1 and −JP2). (C) Fluorescence change by 61 mM [K+] in the BV-infected cells lacking one of essential components (−Cav1.1, −β1a, −Stac3, or −JP2) or with RyR2 cells instead of RyR1. Data are means ± SD (n = 9, N = 3 for 5 BV and n = 6, N = 3 for the others) and were analyzed by one-way ANOVA with Dunnett’s multiple comparisons test. ***, P < 0.0001 from 5 BV. (D) [K+] dependence of R-CEPIA1er fluorescence (F/F0) in cells infected with 5 BV (black), 5 BV plus α2-δ (purple) and 5 BV plus γ1 (orange). Data are means ± SD (n = 9, N = 3 for 5 BV; n = 6, N = 3 for + α2-δ and + γ1). (E) Fluorescence change by 61 mM [K+] in 5 BV, + α2-δ, and + γ1 cells. Data are means ± SD (n = 9, N = 3 for 5 BV; n = 6, N = 3 for + α2-δ and + γ1) and were analyzed by one-way ANOVA with Dunnett’s multiple comparisons test. n is the number of wells and N is the number of independent experiments.](https://cdn.rupress.org/rup/content_public/journal/jgp/154/12/10.1085_jgp.202213230/2/jgp_202213230_fig2.png?Expires=1771745497&Signature=V2Zo2eYyNvSRsLqApuJ1HhEekoJVw6VKTnzKqGyFbnMwALUpT-pYBdac5-i2R55DjmwYFM~jk5s311rPQ0KUT4UO3pxqVGh4cabAWChwUrV-RRpzZd3ZyNuNupj7d3YdnLLv53S57PH~leAP0hqAfNT9O1YgBk-OnwcqHvo1qRLMD~7gFJGhFVmWeJuaMoGNt5rY16WFIUaur4tG2A2958ibNlAUdJpSPecQWGXvfkBmc4J2vw-ACm4HS3fGISHLih~hq2AP9DjpydprU8GSTvYZ3Hvd9qm~CH7YjRXcL76VLI2WGPuJaCYjR2F9euBZFbFZfoyKtCt-mjS4cgReQw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Properties of the reconstituted DICR platform. (A) Typical results of R-CEPIA1er fluorescence in cells infected with 5 BV (left), BV without Cav1.1 (center), and 5 BV plus γ1 (right). (B) [K+] dependence of R-CEPIA1er fluorescence (F/F0) in 5 BV (black), −Cav1.1 (red), and −JP2 (dark blue). Data are means ± SD (n = 9, N = 3 for 5 BV; n = 6, N = 3 for −Cav1.1 and −JP2). (C) Fluorescence change by 61 mM [K+] in the BV-infected cells lacking one of essential components (−Cav1.1, −β1a, −Stac3, or −JP2) or with RyR2 cells instead of RyR1. Data are means ± SD (n = 9, N = 3 for 5 BV and n = 6, N = 3 for the others) and were analyzed by one-way ANOVA with Dunnett’s multiple comparisons test. ***, P < 0.0001 from 5 BV. (D) [K+] dependence of R-CEPIA1er fluorescence (F/F0) in cells infected with 5 BV (black), 5 BV plus α2-δ (purple) and 5 BV plus γ1 (orange). Data are means ± SD (n = 9, N = 3 for 5 BV; n = 6, N = 3 for + α2-δ and + γ1). (E) Fluorescence change by 61 mM [K+] in 5 BV, + α2-δ, and + γ1 cells. Data are means ± SD (n = 9, N = 3 for 5 BV; n = 6, N = 3 for + α2-δ and + γ1) and were analyzed by one-way ANOVA with Dunnett’s multiple comparisons test. n is the number of wells and N is the number of independent experiments.
