Figure S1.
Expression of essential components for DICR machinery using VSV-G pseudotyped BV. (A) Immunofluorescent detection of Cav1.1, β1a, Stac3, JP2, and Kir2.1 in 5 BV (left) and No BV (right) cells. Cells were labeled with antibodies to each component, followed by Alexa594-labeled anti-mouse IgG. Scale bar, 20 μm. Note that red fluorescence was specifically observed with 5 BV cells. (B) Western blots of essential components. Lysates from No BV and 5 BV cells were separated by SDS-PAGE and probed with antibodies to each component. Calnexin (CLNX) was used as a loading control. (C) Western blot of Cav1.1 mutants. Lysates from 5 BV cells carrying WT, R174W, R1086H, T1354S, E100K, F275L, P742Q, and L1367V Cav1.1 were separated by SDS-PAGE and probed with anti-Cav1.1 antibody. Calnexin (CLNX) was used as loading control. Related to Fig. 1 A. Source data are available for this figure: SourceData FS1.

Expression of essential components for DICR machinery using VSV-G pseudotyped BV. (A) Immunofluorescent detection of Cav1.1, β1a, Stac3, JP2, and Kir2.1 in 5 BV (left) and No BV (right) cells. Cells were labeled with antibodies to each component, followed by Alexa594-labeled anti-mouse IgG. Scale bar, 20 μm. Note that red fluorescence was specifically observed with 5 BV cells. (B) Western blots of essential components. Lysates from No BV and 5 BV cells were separated by SDS-PAGE and probed with antibodies to each component. Calnexin (CLNX) was used as a loading control. (C) Western blot of Cav1.1 mutants. Lysates from 5 BV cells carrying WT, R174W, R1086H, T1354S, E100K, F275L, P742Q, and L1367V Cav1.1 were separated by SDS-PAGE and probed with anti-Cav1.1 antibody. Calnexin (CLNX) was used as loading control. Related to Fig. 1 A. Source data are available for this figure: SourceData FS1.

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