Figure S6.
MLC composition and actin filament gliding velocity on nitrocellulose-coated coverslips of myosin extracted from individual type-I M. soleus fibers as a function of the ELC [MLC1sa]/[MLC1sb/v] ratio. (A) MLC SDS-PAGE analysis of individual type-I M. soleus fibers. For each fiber, the respective relative content of MLC1sa (upper band) versus MLC1sb/v (lower band) was determined by densitometric analysis of the Coomassie-stained 15% SDS-PAGE gel. (B) Mean actin gliding velocity of six individual M. soleus fibers as a function of the ELC MLC1sa/MLC1sb/v ratio. A decrease of actin gliding velocity with increasing MLC1sa/MLC1sb/v ratio is reflected by a linear regression to the velocity data with a slope of −0.0392 ± 0.008 (black line). Data points represent mean gliding velocities of individual muscle fibers ± SE. Source data are available for this figure: SourceData FS6.

MLC composition and actin filament gliding velocity on nitrocellulose-coated coverslips of myosin extracted from individual type-I M. soleus fibers as a function of the ELC [MLC1sa]/[MLC1sb/v] ratio. (A) MLC SDS-PAGE analysis of individual type-I M. soleus fibers. For each fiber, the respective relative content of MLC1sa (upper band) versus MLC1sb/v (lower band) was determined by densitometric analysis of the Coomassie-stained 15% SDS-PAGE gel. (B) Mean actin gliding velocity of six individual M. soleus fibers as a function of the ELC MLC1sa/MLC1sb/v ratio. A decrease of actin gliding velocity with increasing MLC1sa/MLC1sb/v ratio is reflected by a linear regression to the velocity data with a slope of −0.0392 ± 0.008 (black line). Data points represent mean gliding velocities of individual muscle fibers ± SE. Source data are available for this figure: SourceData FS6.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal