Figure S5.
Electrophoretic analysis of MLC composition and phosphorylation. (A–C) Imperial protein stain (Thermo Fisher Scientific) SDS-PAGE of the MLCs of skeletal M. psoas and M. soleus, as well as cardiac ventricular and atrial tissue, (B) SyproRuby, PS = PeppermintStick, and (C) SyproRuby color corrected (−25 contrast, 0.22 gamma correction), (D) ProQ, and (E) ProQ color corrected (−25 contrast, 0.22 gamma correction). Protein bands representing the RLCs are found at a molecular weight of ∼19 kD. Source data are available for this figure: SourceData FS5.