Figure 4.
Phosphorylation levels of native tissue-purified M. soleus and ventricular myosin RLCs. Compared to a phosphorylation to protein fluorescence (Pro-Q/SyproRuby) ratio of the positive control (pPS; 45 kD band of the PeppermintStick phosphoprotein standard) of 3.00 ± 0.362 (SE, n = 4), RLCs of M. soleus (MLC2s) and ventricular (MLC2v) myosin showed much lower and comparable ratios of 0.23 ± 0.017 (SE, n = 5) and 0.21 ± 0.018 (SE, n = 5), respectively. The unphosphorylated control protein (upPS; 116 kD band of the PeppermintStick phosphoprotein standard) had a Pro-Q/SyproRuby fluorescence ratio of 0.28 ± 0.094 (SE, n = 4). Neither D/S ratios of M. soleus and ventricular RLCs were significantly different nor ratios of RLCs and of the unphosphorytated control protein. Bars represent mean values ± SE.

Phosphorylation levels of native tissue-purified M. soleus and ventricular myosin RLCs. Compared to a phosphorylation to protein fluorescence (Pro-Q/SyproRuby) ratio of the positive control (pPS; 45 kD band of the PeppermintStick phosphoprotein standard) of 3.00 ± 0.362 (SE, n = 4), RLCs of M. soleus (MLC2s) and ventricular (MLC2v) myosin showed much lower and comparable ratios of 0.23 ± 0.017 (SE, n = 5) and 0.21 ± 0.018 (SE, n = 5), respectively. The unphosphorylated control protein (upPS; 116 kD band of the PeppermintStick phosphoprotein standard) had a Pro-Q/SyproRuby fluorescence ratio of 0.28 ± 0.094 (SE, n = 4). Neither D/S ratios of M. soleus and ventricular RLCs were significantly different nor ratios of RLCs and of the unphosphorytated control protein. Bars represent mean values ± SE.

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