Cholesterol supplementation does not rescue Piezo1 KO NSC proliferation. Quantitation of live-cell imaging of NSC proliferation. Gardner-Altman estimation plot of the fold proliferation after 60 h for Piezo1 KO NSCs with and without cholesterol-MBCD treatment. Data are from four images per three wells for each of n = 15 samples from 7 embryos for WT and n = 15 samples from 7 embryos for Piezo1 KO from eight independent experiments (Cohen’s d = 0.138).