Cerivastatin inhibits NSC proliferation in vitro in a dose dependent manner. (A) Quantification of proliferation from live cell imaging of NSC proliferation as in Fig. 3 E, but with Cerivastatin added at timepoint 0. Data are from four images per each of three wells for each of n = 4 embryos for WT and n = 3 embryos for Piezo1 KO. (B) Quantification of live-cell proliferation in the presence of cerivastatin after 60 h and normalized to untreated NSCs reveals that cerivastatin reduces proliferation of NSCs in a dose-dependent manner. Piezo1 KO NSCs are less sensitive to drug treatment. P values determined by a two-sample t-test.