Oligodendrocyte differentiation is reduced in Piezo1 KO NSCs. Representative fluorescence images of E10.5 WT and Piezo1 KO NSCs differentiated for 10 d in vitro and immunostained for the Oligodendrocyte marker, O4 (magenta) and counterstained nuclei with Hoechst (yellow). n = 6 embryos for WT and n = 4 embryos for Piezo1 KO from two experiments. Related to Fig. 3 C.