Kinetics of EB distribution in the CSF subarachnoid space following high- and low-volume cisternal injections. (A) Left: Images of the brain surface before and 10 and 30 min after cisternal injection of 50 µl of 1% EB in aCSF (high volume) or 5 µl of 10% EB in aCSF (low volume). Right: Average relative intensity (I/I_o) of pixels within the colored circles in images at the left. (B) Left: Initial rate of decline in I/I_o during cisternal EB infusion (*¹, P = 0.0143; *², P = 0.0312; *³, P < 0.00001, t test; n = 4 mice). Right: Maximum percentile change in I/I_o during EB infusion (n.s.¹, P = 0.0678; *², P = 0.0193; *³, P = 0.0115, t test; n = 4 mice). (C) Images of the brain surface (left) and relative intensity (right) during infusion of 5 µl of 10% EB, followed by 50 µl of aCSF.