CaMKII inhibition reduces SCR events in cryoinjured slices from the ENDO. (A and B) Mean values of SCR occurrence and kinetics in LMS from the ENDO in the absence (ncontrol = 4 from four rats; ncryo = 6 from six rats) and presence of AIP (ncontrol = 5 from five rats; ncryo = 8 from seven rats; comparison with two-way ANOVA with Bonferroni post-hoc testing; A), and in the EPI in the absence (ncontrol = 7 from seven rats; ncryo = 5 from five rats) and presence of AIP (ncontrol = 4 from four rats; ncryo = 7 from six rats; comparison with two-way ANOVA with Bonferroni post-hoc testing; B). (C) Mean values of the frequency of spontaneous slice contractions in LMS from the ENDO in the absence (ncontrol = 12 from 11 rats; ncryo = 12 from 10 rats) and presence of AIP (ncontrol = 4 from 4 rats; ncryo = 6 from 6 rats), and from the EPI in the absence (ncontrol = 13 from 12 rats; ncryo = 9 from 9 rats) and presence of AIP (ncontrol = 8 from 7 rats; ncryo = 6 from 5 rats; comparison with Kruskal-Wallis ANOVA with Bonferroni post-hoc testing). (D) Mean values of p-RYR2814/RYR intensity in LMS from the ENDO (ncontrol = 5 from five rats; ncryo = 6 from six rats) and EPI (ncontrol = 5 from five rats; ncryo = 6 from six rats; comparison with Student’s t test). Scale bar, 10 μm.
