SCR events propagate faster and further in a longitudinal direction in cryoinjured ENDO LMSs. (A and B) Mean values of SCR speed (A) and distance propagation in LMS from the ENDO (B; ncontrol = 11 from 11 rats; ncryoinjury = 10 from 10 rats; comparison with Student’s t test) and from the EPI (ncontrol = 10 from 9 rats; ncryoinjury = 7 from 7 rats; comparison with Student’s t test). (C) Direction of propagation of SCR in LMS from the ENDO (ncontrol = 11 from 11 rats; ncryoinjury = 10 from 10 rats) and from the EPI (ncontrol = 10 from 9 rats; ncryoinjury = 7 from 7 rats; comparison with Student’s t test). (D) Mean values of Cx43 localization and Cx43 cluster density in LMS from the ENDO (ncontrol = 7 from six rats; ncryoinjury = 5 from five rats) and from the EPI (ncontrol = 6 from five rats; ncryoinjury = 7 from five rats; Cx43 localization was analyzed using two-way ANOVA with Bonferroni post-hoc testing; Cx43 cluster density was analyzed using Student’s t test).
