Cryoinjury of LMS induces local remodeling in the region adjacent to the injury. (A) Examples of LMS during different stages of preparation. A freshly cut LMS is trimmed according to the fiber orientation. The trimmed LMS is attached to holders using tissue glue. After attachment to the holders, cryoinjury was induced using a 3-mm ice-cold cylindrical rod. Lastly, the LMS is positioned onto a stretcher to allow mechanical loading. The region adjacent to the cryoinjury (<2 mm) was studied to investigate SCR events in regions of the LV wall. (B) Examples and mean values of TATS in LMS from the ENDO (n_control = 11 from 11 rats; n_cryoinjury = 9 from 9 rats; comparison with Student’s t test) and from the EPI (n_control = 11 from 10 rats; n_cryoinjury = 9 from 8 rats; comparison with Student’s t test). (C) Examples and mean values of kinetics of Ca²⁺ transients at 1-Hz pacing in LMS from the ENDO (n_control = 14 from 12 rats; n_cryoinjury = 12 from 12 rats; comparison with Student’s t test) and from the EPI (n_control = 10 from 9 rats; n_cryoinjury = 15 from 15 rats; comparison with Student’s t test).