Effects of CBD on rat diaphragm contraction. (a) Image of dissected rat diaphragm muscle. (b) Image of rat diaphragm cut into a hemidiaphragm, which was placed between electric plates that were used for electric stimulation. The subsequent muscle contractions were measured using a force transducer. (c) Normalized quantification of muscle contractions in CBD and TTX (percentage of normalized contraction: control = 100 ± 5.3%, n = 9; CBD = 60.6 ± 3.5%, n = 8; TTX = 28.9 ± 5.3%, n = 6; * indicates P = 0.0006 for CBD; # indicates P < 0.0001 for TTX). Error bars are SE in mean. (d–f) Sample contraction traces across all three conditions.