AP simulations of skeletal muscle APs in the presence and absence of CBD, based on voltage-clamp data. Top of the figure shows the pulse protocol used for simulations, and a cartoon representation of P1158S-pH in vitro/in silico assay, where pH can be used to control the P1158S phenotype. (a and b) Simulations in WT-Nav1.4 in the presence and absence of CBD. (c and d) Simulations of P1158S at pH6.4. (e and f) Results from pH 7.4.