Structural integrity MD simulation of CBD pathway through the Nav1.4 fenestration. Root mean square deviation (RMSD) of the fenestration residues as a function of time in the absence (black) and the presence of CBD passing through the fenestration (red and green, two different simulation parameter sets). Apo means unbound in the absence of CBD. The similar RMSD profiles show that CBD’s passage does not distort the structural integrity of the fenestration.