Intensity of reflections along the meridian from XRD patterns of relaxed tarantula skeletal muscle. Integrated area seen in Fig. 2 A, orange box. (A and B) Ex vivo leg muscles (A) and isolated live muscle (B). (C and D) Radial intensity profile of the third myosin reflection M3 (C) and normalized (D) at <12.5°C, 22.5°C, and >33.5°C. (E) Radial width of the M3 reflection for <12.5°C and 22.5°C. The results are given as mean ± SEM. ns, not significant.