Variation of the intensities of layer lines and meridional and equatorial reflections and their spacings in live relaxed tarantula skeletal muscle with temperatures (t) from 8.5°C to 40°C. (A) Normalized I_MLL4. (B–D) Normalized I_1,0 (B) and I_1,1+2,0 (C) and the change of the I_1,1+2,0/I_1,0 ratio ΔI_1,1+2,0/I_1,0 (D). (E–H) Normalized intensities of the third (E) and sixth (F) meridional reflections (M3 and M6, respectively) and their relative spacing changes (G and H). The residual values of I_MLL4 are shown in A with black (20%) and white (40%) arrows. The average value and its SEM is shown for each measurement at each temperature. Normalization was against values measured at 22.5°C. The results are given as mean ± SEM.