Figure S7. Mutations of Ca 2 site near... | Rockefeller University Press

Figure S7.

Mutations of Ca2+ site near the dimer interface do not alter pHi regulation on TMEM16F.(A and B) Representative TMEM16F-D859A and TMEM16F-E395A currents recorded from inside-out patches perfused with intracellular solutions containing 100 µM Ca2+ at different pHi values. (C and D) The G-V curves of D859A and E395A currents, respectively. Error bars represent SEM (n = 5). (E) The pHi sensitivity of D859A (red) and E395A (blue) evaluated by the G-pHi relationship. The G-pHi curves of WT at different Ca2+ concentrations were also plotted as dashed lines (black) for reference.

Mutations of Ca²⁺ site near the dimer interface do not alter pH_i regulation on TMEM16F. (A and B) Representative TMEM16F-D859A and TMEM16F-E395A currents recorded from inside-out patches perfused with intracellular solutions containing 100 µM Ca²⁺ at different pH_i values. (C and D) The G-V curves of D859A and E395A currents, respectively. Error bars represent SEM (n = 5). (E) The pH_i sensitivity of D859A (red) and E395A (blue) evaluated by the G-pH_i relationship. The G-pH_i curves of WT at different Ca²⁺ concentrations were also plotted as dashed lines (black) for reference.