Figure S5. TMEM16A-CaCC loses pH i... | Rockefeller University Press

Figure S5.

TMEM16A-CaCC loses pHi regulation under saturating Ca2+.(A) Representative TMEM16A currents recorded from inside-out patches perfused with intracellular solutions containing 100 µM Ca2+ at different pHi values. (B) G-V relationships at different pHi values under 100 µM Ca2+. All conductances were normalized to the maximum conductance at pHi 8.9 and +100 mV. Error bar represents SEM (n = 5). (C) G-pHi curve of TMEM16A at 100 µM Ca2+ (red solid line) and 0.5 µM Ca2+ (black dotted line). The slopes from linear fits are 0.02 and 0.3, respectively. Error bar represents SEM (n = 5).

TMEM16A-CaCC loses pH_i regulation under saturating Ca²⁺. (A) Representative TMEM16A currents recorded from inside-out patches perfused with intracellular solutions containing 100 µM Ca²⁺ at different pH_i values. (B) G-V relationships at different pH_i values under 100 µM Ca²⁺. All conductances were normalized to the maximum conductance at pH_i 8.9 and +100 mV. Error bar represents SEM (n = 5). (C) G-pH_i curve of TMEM16A at 100 µM Ca²⁺ (red solid line) and 0.5 µM Ca²⁺ (black dotted line). The slopes from linear fits are 0.02 and 0.3, respectively. Error bar represents SEM (n = 5).