Figure 9.
GxTX-594 labels CA1 hippocampal pyramidal neurons transfected with Kv2.1-GFP.(A) Two-photon excitation images of fluorescence from the soma and proximal dendrites of a rat CA1 hippocampal pyramidal neuron in a brain slice 6 d after transfection with Kv2.1-GFP (left), labeled with 100 nM GxTX-594 (middle), and the overlay (right). Image represents a Z-projection of 20 optical sections. Scale bar, 10 μm. (B) A single optical section of the two-photon excitation image shown in A. GxTX-594 labels both Kv2.1-GFP puncta from a transfected cell and apparent endogenous Kv2 proteins from an untransfected cell in the same cultured slice (right, arrow). Scale bar, 10 μm. (C) Pearson correlation coefficients from CA1 hippocampal neurons 2, 4, and 6 d after transfection with Kv2.1-GFP. Each circle represents a different neuron. Bars are arithmetic means.

GxTX-594 labels CA1 hippocampal pyramidal neurons transfected with Kv2.1-GFP. (A) Two-photon excitation images of fluorescence from the soma and proximal dendrites of a rat CA1 hippocampal pyramidal neuron in a brain slice 6 d after transfection with Kv2.1-GFP (left), labeled with 100 nM GxTX-594 (middle), and the overlay (right). Image represents a Z-projection of 20 optical sections. Scale bar, 10 μm. (B) A single optical section of the two-photon excitation image shown in A. GxTX-594 labels both Kv2.1-GFP puncta from a transfected cell and apparent endogenous Kv2 proteins from an untransfected cell in the same cultured slice (right, arrow). Scale bar, 10 μm. (C) Pearson correlation coefficients from CA1 hippocampal neurons 2, 4, and 6 d after transfection with Kv2.1-GFP. Each circle represents a different neuron. Bars are arithmetic means.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal