Figure 5.
GxTX-594 fluorescence equilibration after dilution from 100 nM to 9 nM.(A) CHO cells transfected with Kv2.1-GFP (left) in 100 nM GxTX-594 (middle) and after dilution to 9 nM GxTX-594 (right). Regions darker than the background bath solution are CHO cells not transfected with Kv2.1-GFP (arrowhead). Scale bar, 20 μm. (B) Fluorescence excited at 594 nm during dilution from 100 nM GxTX-594 to 9 nM GxTX-594. Timing of dilution is shown above the graph. Fluorescence was normalized to the mean 594 fluorescence before dilution to 9 nM. Data are from the cell labeled with an arrow in A. 100 nM and 9 nM images in A are from 0 and 12 min, respectively. (C) Rate of GxTX-594 fluorescence decay after dilution. Error bars are SDs of kΔF fit. n = 5 cells.

GxTX-594 fluorescence equilibration after dilution from 100 nM to 9 nM. (A) CHO cells transfected with Kv2.1-GFP (left) in 100 nM GxTX-594 (middle) and after dilution to 9 nM GxTX-594 (right). Regions darker than the background bath solution are CHO cells not transfected with Kv2.1-GFP (arrowhead). Scale bar, 20 μm. (B) Fluorescence excited at 594 nm during dilution from 100 nM GxTX-594 to 9 nM GxTX-594. Timing of dilution is shown above the graph. Fluorescence was normalized to the mean 594 fluorescence before dilution to 9 nM. Data are from the cell labeled with an arrow in A. 100 nM and 9 nM images in A are from 0 and 12 min, respectively. (C) Rate of GxTX-594 fluorescence decay after dilution. Error bars are SDs of kΔF fit. n = 5 cells.

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