Figure S8.
Effect of cytoskeleton manipulation on the membrane diffusion of PIEZO1-GFP.(A) Quantification of lateral unbinding rate (s−1) of PIEZO1-GFP entities in control and after treatments. (B) Effective diffusion (µm2/s) of PIEZO1-GFP entities in control and after treatments. Disruption of the cellular cytoskeleton has no effect on the dispersion rate and the lateral unbinding rate of PIEZO1-GFP clusters, while reinforcement reduces its micrometer-scale diffusion rates. CytoD, cytochalasin D (n = 5 trials, 170 cells); Jasp, jasplakinolide (n = 5 trials, 79 cells); Colch, colchicine (n = 5 trials, 104 cells). One-way ANOVA, *, P < 0.05; n.s., not significant; error bars represent SEM.

Effect of cytoskeleton manipulation on the membrane diffusion of PIEZO1-GFP. (A) Quantification of lateral unbinding rate (s−1) of PIEZO1-GFP entities in control and after treatments. (B) Effective diffusion (µm2/s) of PIEZO1-GFP entities in control and after treatments. Disruption of the cellular cytoskeleton has no effect on the dispersion rate and the lateral unbinding rate of PIEZO1-GFP clusters, while reinforcement reduces its micrometer-scale diffusion rates. CytoD, cytochalasin D (n = 5 trials, 170 cells); Jasp, jasplakinolide (n = 5 trials, 79 cells); Colch, colchicine (n = 5 trials, 104 cells). One-way ANOVA, *, P < 0.05; n.s., not significant; error bars represent SEM.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal