Changes in the F.I. of RT nanosheets with HeLa cells before and after treatment with trypsin, PFA, or Triton X-100. (A) Time course of changes in the F.I. of EuTTA (left), Rh101 (middle), and the EuTTA/Rh101 ratio of RT nanosheets upon trypsin (final 0.12%) treatment. Gray lines, individual cells; red lines, average of individual cells; blue lines, average without cells. (B) Same as in A with the F.I. corrected by that without cells. See Materials and methods for details. Gray lines, individual cells; red lines, average of individual cells. In A and B, the horizontal black line indicates the period for trypsin treatment. Number of cells, 12 (n = 3). (C) Same as in A upon PFA (final 4%) treatment. (D) Same as in C with the F.I. corrected by that without cells. In C and D, the horizontal black line indicates the period of PFA treatment. Number of cells, 21 (n = 3). (E) Same as in A upon Triton X-100 (final 0.9%) treatment for fixed HeLa cells with PFA. (F) Same as in E with the F.I. corrected by that without cells. In E and F, the horizontal black line indicates the period of Triton X-100 treatment. Number of cells, 14 (n = 3).