Ion flux through KW/GD(G) open conducting structures. (A) Left: Average (±SD) ion occupancy along the pore was computed over the resampled simulations after excluding 500, 400, 200, and 0 ns from the concatenated 2.8- and 3-µs simulations of KW/GD and KW/GD(G), respectively, from which the average apparent free energy profile for potassium ions along the pore axis was obtained (right) via an inverse Boltzmann transformation of the K⁺ ion density profile obtained under the influence of applied electric field. (B) Estimated conductance from individual KW/GD and KW/GD(G) simulations. (C) Single-channel currents recorded from CosM6 cells at −120 mV and 150 mM symmetrical K⁺ condition using inside-out patch configuration at pH 7.5. This figure is representative of six single-channel recordings of WT Kir2.2 and KW/GD channels (combined duration of >3 min for each). (D) Average single-channel current–voltage relationships (of four or more patches excised from different cells in each case); error bars (where visible) represent mean ± SEM of values obtained from individual records.