Figure 4.
Regulation of endogenous SK currents in hiPSC-CMs by intracellular CaM peptides.(A) hiPSC-CMs expressing SK2 channels (green) and α-actinin2 (red). 4′,6-Diamidino-2-phenylindole stain is shown in blue. Scale bar is 10 µm. (B) Effects of mutant CaM peptides (CaMN54I, CaMF90L, and CaMD96V) compared with CaMWT peptide on apamin-sensitive SK currents in hiPSC-CMs. The current was recorded before (black trace) and after (red trace) apamin application (100 nM). (C) Subtracted apamin-sensitive SK currents in the presence of CaMWT and mutant CaM peptides. (D) Comparisons of the apamin-sensitive current densities at −120 mV and +60 mV (n = 8–10 cells). Data represent mean ± SEM. Statistical analyses were performed using one-way ANOVA combined with Tukey’s test; *, P = 0.012 at −120 mV, and *, P = 0.037 at +60 mV between CaMWT and CaMD96V.

Regulation of endogenous SK currents in hiPSC-CMs by intracellular CaM peptides. (A) hiPSC-CMs expressing SK2 channels (green) and α-actinin2 (red). 4′,6-Diamidino-2-phenylindole stain is shown in blue. Scale bar is 10 µm. (B) Effects of mutant CaM peptides (CaMN54I, CaMF90L, and CaMD96V) compared with CaMWT peptide on apamin-sensitive SK currents in hiPSC-CMs. The current was recorded before (black trace) and after (red trace) apamin application (100 nM). (C) Subtracted apamin-sensitive SK currents in the presence of CaMWT and mutant CaM peptides. (D) Comparisons of the apamin-sensitive current densities at −120 mV and +60 mV (n = 8–10 cells). Data represent mean ± SEM. Statistical analyses were performed using one-way ANOVA combined with Tukey’s test; *, P = 0.012 at −120 mV, and *, P = 0.037 at +60 mV between CaMWT and CaMD96V.

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